Virus:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Recombinant:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Staining:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Cell Isolation:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
CCK-8 Assay:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Enzyme-linked Immunosorbent Assay:Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Histone 3 Bioss Catbs-17422R Rabbit anti-C1QC Bioss Cat#bs-11336R Rabbit anti-H2B Bioss Cat#bs-52009R Rabbit anti-C3B Bioss Cat#bs-4871R Rabbit anti-GAPDH Bioss Cat#bs-2188R;RRID:AB_10856675 Rabbit anti-b-tubulin Bioss Cat#bs-0715R;RRID:AB_10853042 Rabbit anti-CD8 Bioss Cat#bs-4790R Rabbit anti-Granzyme B Bioss Cat#bs-1351R;RRID:AB_10856802 Rabbit anti-IRAK4 Bioss Cat#bs-2440R;RRID:AB_11067218 Rabbit anti-TRAF6 Bioss Cat#bs-2830R Rabbit anti-TAK1 Bioss Cat#bs-3585R;RRID:AB_10855725 Rabbit anti-Siglec15 KleanAB Cat#P103014 Anti-mouse RANK-PE Biolegend Cat#119805;RRID: AB_2205354 Anti-mouse CD3e eBioscience Cat#16-0033-86;RRID:AB_842782 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074;RRID:AB_2099233 Bacterial and virus strains Lentivirus-luc-gfp-puro HanBio https://www.hanbio.cn/ cn/services/item/1 N/A Biological samples Human Primary CD8+ T cells MEISEN CELL Cat#CTCC-009-287 Chemicals, peptides, and recombinant proteins Biotin MAL-II Vector Laboratories Cat#B-1265-1 Biotin SNA Vector Laboratories Cat#B-1305-2 FITC-Streptavidin Biolegend Cat#405201 Recombinant mouse RANKL R&D Systems Cat#462-TR-010 Recombinant mouse M-CSF R&D Systems Cat#416-ML-050 Recombinant mouse Siglec15 R&D Systems Cat#5A4074-M31H Staurosporine MedChemExpress Cat#HY-15141 Microspheres Aladdin Cat#M122073/M122077 Alendronate sodium Aladdin Cat#A115345 Sialidase Sigma-Aldrich Cat#11585886001 D-luciferin salt solution Meilunbio Cat#MB1834-2 5,6-carboxyfluorescein diacetate succinimidyl ester Thermo Scientific Cat#C34570 60-sialic acid lactose sodium salt MACKLIN Cat#S868592 Critical commercial assays TRAP stain kit Sigma-Aldrich Cat#CS0740 Annexin V and PI Apoptosis Kit US Everbright Cat#F6012 EasySep Mouse Naive CD8+ T cell Isolation Kit StemCell Technologies Cat#19858 Cell Counting Kit-8 Beyotime Biotechnology Cat#C0038 Mouse IFN-g Elisa Kit FankeW Cat#F2182-A Mouse TNF-a Elisa Kit FankeW Cat#F2132-A Mouse IL-2 Elisa Kit FankeW Cat#F2698-A (Continued on next page) e1 Cell Reports Medicine 4, 101165, September 19, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Human IFN-g Elisa Kit 4A Biotech Cat#CHE0017 RNA-Quick Purification Kit YiShanBiotech Cat#ES-RN001 BCA protein assay Kit Beyotime Biotechnology Cat#P0010 DAB Chromogenic Kit Beyotime Biotechnology Cat#P0203 Mouse Spleen Lymphocyte Isolation Kit Solarbio Cat#P8860 IHC Detection Reagent Cell Signaling Technology Cat#8114 TSA Fluorescent Triple Staining Kit Servicebio Cat# G1236 Deposited data Raw data and processed bulk RNA-seq data(mouse) GEO GEO:GSE133515/GSE132230 Siglec15-interacting proteins ProteomeXchange Consortium PXD:006359 Experimental models: Cell lines 4T-1 Procell Cat #CL-0007 EMT-6 Procell Cat #CL-0573 THP-1 Procell Cat#CL-0233 HEK293 Procell Cat#CL-0005 MA-891 iCellBioscience Cat#iCell-m029 Experimental models: Organisms/strains Mouse: C57BL/6J Laboratory Animal Center of Third Military Medical University N/A Mouse: BALB/c Laboratory Animal Center of Third Military Medical University N/A Mouse: MRL/lpr The Jackson Laboratory JAX:000485 Mouse:Siglec15 / SHANGHAI MODEL ORGANISMS NM-KO-191192 Oligonucleotides Siglec15 primer forward 50- CAGCACCGAGATGTTGACGA-30 TsingkeBiotechnology N/A Siglec15 primer reverse 50- ACGATCGCTATGAGAGTCGC-3’.
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